Immunoblot of plasma with monoclonal antibodies HKH 14 and HKH 15. Pooled normal human plasma (NHP), plasmas from the family members (1:20 in TBS), and plasmas from the propositus, Fitzgerald (FITZ), and Williams (1:10) were electrophoresed on a reducing 7% SDS-PAGE, followed by electroblotting onto nitrocellulose and incubation with monoclonal antibodies HKH14 (panel A) and HKH15 (panel B) at 2 μg/mL in Blotto.²³  Bound antibody was detected by peroxidaseconjugated secondary antibody to mouse IgG followed by chemiluminescence.