Immunoblotting of plasma with polyclonal antibody directed to the light chain of HK. Pooled normal human plasma (NHP) and plasmas from the family members were diluted 1:20 in TBS prior to the addition of an equal volume of sample buffer. Plasmas from the propositus, Fitzgerald (FITZ), and Williams were diluted 1:10. All samples were reduced with 2% β-mercaptoethanol and boiling prior to being applied to a 7% SDS-PAGE. After electrophoresis, the samples were electroblotted onto nitrocellulose and incubated with polyclonal antibody AHMWK3 at 1:500 in Blotto.23 Bound antibody was detected by peroxidase-conjugated secondary antibody to goat immunoglobulin G (IgG) followed by chemiluminescence. The figure is a representative immunoblot of 3. In this and subsequent figures, the numbers on the left represent molecular mass standards in kilodaltons.