Northern and Western blot of AML-1, c-myb, CDP, CASP, GATA-1, and glycophorin-A. (A) Northern blot of total RNA from mature neutrophils and the 3 MACS-depleted populations of neutrophil precursors. The blot was hybridized with probes against AML-1, c-myb, CDP, GATA-1, and glycophorin-A. CASP is a splice variant of CDP. RNA from erythrocytes (ERY) was included as a positive control for glycophorin-A and GATA-1. (B) Schematic representation of the hybridization intensities from panel A. For each probe, the cell population showing maximal expression is given the value 1; the expression levels in the other cell populations are shown relative to this. All expression levels are mean values of 18S-normalized transcript levels from 3 different subjects. Standard deviations (SDs) are shown as error bars. (C) Western blot of AML-1, c-myb, and CDP. Equal loading was assessed by probing with an antibody against α-tubulin. The arrows indicate the bands of the target proteins.