CD8⁺ cells do not alter the integrity of the endothelial barrier.

Chemotaxis assays were performed as described with the addition of fluorescently labeled albumin. Movement of labeled albumin into the bottom of the Transwell was used as a surrogate for the integrity of the BMEC layer measuring the ability of albumin to pass through the cell-coated Transwell in the presence or absence of the indicated nonadherent cells. The data are represented as the means of 3 independent experiments; error bars represent the SEMs.