Evidence for TSHR expression in murine BM.

(A) RT-PCR analyses of TSHR in whole BM cells indicates active expression of the TSHR gene. (B) Two-color flow cytometric analyses of BM cells in regions R1, R2, and R3 (Figure 1) stained with biotinylated recombinant TSHβ and for identification of TSHR⁺ cells and anti-CD11b indicate a preponderance of TSHR⁺ cells among the lymphocyte precursor population; the overall lack of TSHR staining for Ter-119 cells indicates that cells in the region R1 are not erythrocyte precursors. Data are representative of 2 to 4 experiments.