PCs with both the normal and abnormal pattern of hybridization. The depicted PCs show normal (A) and abnormal (B) patterns of hybridization. All panels show the blue fluorescence of the cytoplasm allowing the clone-specific interphase FISH scoring. (A) A cell with the normal configuration of 2 pairs of signals for the probes localizing to the centromere 17 (CEP17; green) and the 17p13.1 (LSI p53) (red) probe. (B) A cell with deletion of 17p13.1. There are 2 green signals arising from the centromeric probe but only 1 red signal from the p53 locus probe. (C) A normal configuration of probes used to detect the t(14;16)(q32;q23). The locus-specific 14q32 probes are labeled in green, and the 16q23 probes are labeled in red. (D) A cell with fusion of probes for 14q32 (green) and 16q23 (red). The 2 signals in proximity generate a fusion. If a significant number of cells scored showed this pattern, a patient is said to have a translocation.