Figure 7.
Figure 7. Proliferation and differentiation of CD79a+-enriched CDw127+CD11b–CD36–CD19– cells cultured at limiting dilution. Plates (96 wells) precoated with MS-5 cells were seeded with either single cells (n = 288 wells, black bars) or 10 cells (n = 192 wells, dotted bars) in lymphoid conditions (see “Materials and methods” for details). Plates were grown for 14 days, and the number of nucleated cells in each was estimated by microscopic examination. Bars show the proportion of total wells containing the estimated number of nucleated cells indicated on the x-axis. The inset table shows the proportion of wells that contained cells from 2 or more lineages. Data are from 36 wells seeded with single cells and 26 wells seeded with 10 cells.

Proliferation and differentiation of CD79a+-enriched CDw127+CD11bCD36CD19 cells cultured at limiting dilution. Plates (96 wells) precoated with MS-5 cells were seeded with either single cells (n = 288 wells, black bars) or 10 cells (n = 192 wells, dotted bars) in lymphoid conditions (see “Materials and methods” for details). Plates were grown for 14 days, and the number of nucleated cells in each was estimated by microscopic examination. Bars show the proportion of total wells containing the estimated number of nucleated cells indicated on the x-axis. The inset table shows the proportion of wells that contained cells from 2 or more lineages. Data are from 36 wells seeded with single cells and 26 wells seeded with 10 cells.

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