Fig. 7.
![Fig. 7. Rapamycin does not affect apoptosis in cycling or resting B-CLL cells. / (A) Annexin V and PI staining were performed in B-CLL cells after 48 or 72 hours of culture in the presence of medium alone or DSP30 and IL-2 (stimulated/activated[stim]) with or without rapamycin (Rap). Viable cells were annexin- and PI-negative. Results were obtained from 5 different samples and are presented as means ± SEMs. (B) For TUNEL staining, cells were cultured for 72 hours using the same conditions as described above. One additional independent experiment gave similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/1/10.1182_blood-2002-01-0189/6/h80133615007.jpeg?Expires=1724265197&Signature=dhG8mq6IpQw6OEBmFgd3iz4U1dOOLN0eM9~WclT8E~lnC94A3rUqtNwMu95X6zYapz6pFHiYTvYCs65n3I5PdLN-5xgrPZndkD77wu1EPWNBmWhzeeny7Xp~jvENJW-jxhLeB5UAXBlzsAudb1zQNjWXULq5XampEt4ASF6zXPHriXh~Z6b4EX3m2zg1nPw5V-1S1UcNja-5yrPtSh3cV1OfEEZGpORRroaXFJk-ud~NqarmDc~FcZd5SybE57aPt6GsXeOuKqDn~UwAguBCqXjQ94PY9PlKNWx9gsCSNsfQN-nxGCdui8HXM9Dtmgj-jaU0GULxAQoCQrv6NmrDHA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Rapamycin does not affect apoptosis in cycling or resting B-CLL cells.
(A) Annexin V and PI staining were performed in B-CLL cells after 48 or 72 hours of culture in the presence of medium alone or DSP30 and IL-2 (stimulated/activated[stim]) with or without rapamycin (Rap). Viable cells were annexin- and PI-negative. Results were obtained from 5 different samples and are presented as means ± SEMs. (B) For TUNEL staining, cells were cultured for 72 hours using the same conditions as described above. One additional independent experiment gave similar results.
