NK cell–mediated induction of apoptosis in breast carcinoma cells.

SKBR3 cells were incubated for one hour with NK-92-scFv(FRP5)-ζ (A, D) or parental NK-92 cells (B, E) at an E/T ratio of 3:1. Control cells were cultured in the absence of NK cells (C, F). Apoptotic cells were labeled with fluorescein using the TUNEL method and visualized under a fluorescence microscope with blue light excitation. Fluorescent images of representative fields are shown in (A) to (C); phase contrast images of the same sections are shown in (D) to (F).