Fig. 2.
Northern blot analysis of IL-7R gene expression.
Total RNA (20 μg/lane) from HS-27a, HS-5, or PBMCs was separated on an RNA agarose gel and blotted onto a nylon membrane. The membrane was probed with a P32-labeled IL-7R probe (A). The DNA sequence of the probe covers 1098 base pairs from the 3′ end and was confirmed to hybridize to human IL-7R. The bound probe was stripped off, and the membrane was reprobed with a P32-labeled actin probe (B). The data indicate a strong signal for IL-7R sequences in RNA isolated from HS-27a and PBMCs but not HS-5 cells.
