Expression of the MSCV-HS2-β-globin-GFP vector human β-globin transgene in the erythroid progeny of transduced human fetal liver cells.

RNA was extracted from cells harvested from the marrow of mice engrafted with transduced human fetal liver cells (E1-5) and control mice engrafted with mock-transduced human fetal liver cells (C1,2) and from 3-week cultures of human erythroid cells derived from these mice. The presence of transgene-specific β-globin messenger RNA was assessed by RT-PCR using primers that amplify the mutant sequence of the β-globin transgene (Figure 1) not present in the normal human β-globin gene.