Correction of aberrant splicing by U7.623 snRNA in β-globin IVS2 mutant cells.

Total RNA from HeLa β-globin IVS2 mutant cells transiently transfected with 0.05 to 1.0 μg (indicated at the top) U7.623 snRNA plasmid was analyzed by RT-PCR. (A) β-Globin IVS2-654, (B) β-globin IVS2-705, and (C) β-globin IVS2-745 HeLa cells. Lane 1, untreated cells; lanes 2 to 5, cells transfected with U7.623 plasmid; lane 6, (N) RNA from normal human blood. The sizes (in nucleotides) of PCR bands representing aberrantly (308, 358, and 396) and correctly (231) spliced mRNAs are indicated on the right. Similar designations were used in Figures 5, 6C, and 7A.