Fig. 2.
Fig. 2. Correction of aberrant splicing by modified U7 snRNAs in EGFP-705U HeLa cells. / (A) FACS analysis of HeLa EGFP-705U cells transiently transfected with 0.05 to 1.0 μg U7 snRNA plasmids (indicated on the left). (B) Cells transfected with U7.623, U7.324, and control U7.SmOPT (3 independent experiments) were analyzed in terms of FI (percentage of cells scoring above the background threshold × mean fluorescence intensity of that cell subpopulation).

Correction of aberrant splicing by modified U7 snRNAs in EGFP-705U HeLa cells.

(A) FACS analysis of HeLa EGFP-705U cells transiently transfected with 0.05 to 1.0 μg U7 snRNA plasmids (indicated on the left). (B) Cells transfected with U7.623, U7.324, and control U7.SmOPT (3 independent experiments) were analyzed in terms of FI (percentage of cells scoring above the background threshold × mean fluorescence intensity of that cell subpopulation).

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