The effects of MMP-cleavage of MCP-2 and MCP-4 on THP-1 monocytic cell chemotaxis.

(A) (B) Cell migration assay of THP-1 cells in response to MCP-2 and MCP-2(5-76) (panel A) or MCP-4 and MCP-4(8-75) (panel B), as indicated. Cells migrated in the absence of chemokine are shown at 0 M chemokine. Mean values of duplicate assays are presented. (C) (D) Antagonist assays are shown for MCP-2(5-76) (panel C) and MCP-4(8-75) (panel D). Chemotaxis of THP-1 cells in response to 30 nM MCP-2 or 100 nM MCP-4 in the absence and presence of MMP-cleaved chemokine analog at the indicated concentrations is shown in the corresponding panels. Mean values of duplicate assays are presented.