Receptor activity of full-length MCP-4 and MCP-4(8-75).

(A) (B) Cell migration assays of B300-CCR2 (panel A) or B300-CCR3 (panel B) showing activity of MCP-4(8-75), MCP-4, or vehicle alone (0 M). (C) Cell migration assay of B300-CCR2 showing, as indicated, MCP-4 (300 nM) in the presence of increasing concentrations of MCP-4(8-75) or MCP-4 alone—ie, with 0 M MCP-4(8-75). (D) Cell migration assay of B300-CCR3 in response, as indicated, to full-length MCP-4 (100 nM) and in the presence of increasing concentrations of MCP-4(8-75) or MCP-4 alone, ie, with 0 M MCP-4(8-75). Mean values of duplicate assays are shown.