Receptor activity of full-length MCP-2 and MCP-2(5-76).

(A) Cell migration assays of B300-CCR2 showing loss of receptor agonist activity of MCP-2(5-76) compared with full-length chemokine. Cells that migrated in the absence of chemokine are indicated at 0 M. (B) Cell migration assay of B300-CCR2 in the presence of full-length MCP-2 (30 nM) and phosphate-buffered saline vehicle alone (0 M) or increasing concentrations of MCP-2(5-76), as indicated. Mean values of duplicate assays are shown.