MMP cleavage of MCP-1, MCP-2, and MCP-4.

Tris-tricine gel analysis and masses obtained by electrospray ionization mass spectrometry of MCP-1, MCP-2, and MCP-4 cleavage products produced by MMP activity. APMA-activated MMP (1 ng) was incubated with 1 μg MCP in CAB buffer at 37°C for 8 hours. Reaction was stopped by the addition of SDS-PAGE sample buffer. The mass of the molecular weight markers is indicated.