Specificity of MMP cleavage of MCP-3.

Tris-tricine gel analysis and masses obtained by electrospray ionization mass spectrometry of MCP-3 cleavage products produced by MMP activity. APMA-activated MMP (1 ng) was incubated with 1 μg MCP-3 in CAB assay buffer at 37°C for 8 hours. Aliquots were taken for mass spectroscopic analysis, and the reaction was stopped by the addition of SDS-PAGE sample buffer prior to electrophoresis. The mass of the molecular weight markers is indicated.