Effect of soluble mediators on the maturation and cell survival of MoDCs in the absence of growth factors and presence of human serum.

On day 7, immature MoDCs cultures were washed and resuspended in fresh medium containing GM-CSF (GM, 20 ng/mL). Proinflammatory factors with TNF-α (10 ng/mL), IFN-α (2000 IU/mL), and PGE₂ (1 μM) were added for 3 days. On day 10, cells were washed and recultured in either IMDM + 5% human serum alone or with CD40L (1 μg/mL). MoDCs from these conditions were assessed by Annexin V and PI labeling for death by apoptosis using FACS. Data are representative of experiments from 5 separate donors.