T-cell stimulation by immature and mature MoDCs.

Immature (GM + IL-4) or MoDCs (1 × 10⁴) matured with the indicated stimuli were washed and used as stimulators of alloreactive T cells (1 × 10⁵) in the MLR. On day 5 of the MLR, supernatants were harvested and fresh medium containing 1 μCi (0.037 MBq) [³H]thymidine was added into each well for 8 hours. Cytokines secreted by naive CD4⁺, CD45RA⁺ alloreactive T cells following stimulation with DCs were also assessed by ELISA. (A) IFN-γ production. (B) IL-2 production. (C) IL-5 production. Data represent the means ± SEM of experiments from 3 separate donors. (D) Proliferation of T cells stimulated with graded numbers of MoDCs. Data represent the means ± SEM of triplicate wells. The figure is representative of experiments from 5 separate donors.