Evaluation of the naive and memory profile of T cells in WAS patients after HST.

Density plots from 2-color immunofluorescence depict the results of 3-color FCM-WASP using anti-CD45RA or anti-CD45RO for each population of CD4⁺ and CD8⁺ T cells. All cells pictured were gated with a forward- and side-scatter lymphocyte gate combined with a CD4⁺ or CD8⁺ gate. The x-axis represents WASP staining intensity; the y-axis represents CD45RA or CD45RO expression. Numbers within the density plots represent the percentage of cells in each quadrant. It is noteworthy that in contrast to the WASP^bright donor CD4⁺ and CD8⁺cells, most WASP^dim recipient cells in patients 1, 2, and 3 remained CD45RA⁺/CD45RO⁻ (naı̈ve) at the indicated days after HST.