![Fig. 1. Pictures with both the normal and abnormal patterns for the break-apart and fusion strategies. / In all cases the clonal PCs are identified by the intense blue fluorescence of the cytoplasm as detected by the cIg-FISH procedure. (A) A PC with the normal configuration for the VH (red signal) and CH probes (green signal). Arrows point to the closely associated pairs of signals indicative of a normal pattern. (B) Segregation (break-apart) of the VH (green) and CH (red) probes consistent with a 14q32 translocation. (C) A cell with fusion signals (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 4p16.3 (red) consistent with a t(4;14)(p16.3;q32). (D) The fusion signal (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 16q23 (red) consistent with a t(14;16)(q32;q23). (× 100 magnification, Leica DMRXA microscope [Wetzlar, Germany]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/4/10.1182_blood.v100.4.1417.h81602001417_1417_1424/6/h81622989001.jpeg?Expires=1723709043&Signature=kwckKyGeJjrym3YnLeMI1d5WoziBT7R~MnfS5dnC1ph98Ri0hyCqI-ZMC0DSG3g8iyKgTyfJsQmm47Bmu4KMUJUNtjj-VOijaSCQi5O7z0MCaxoS3M~HD14heSka9oso10RBT0~ZRahEAeX5F4Hd66sX~a1YNqs8mYop41id0E4y5DOv5l1maAlWbHvN81ujXq23z5HZ0jx1zwzzA1UWDdVRrUzR3LPkPK0sjer3VV1Kdkkt1mex69Zbqg4N~7S-kpqviisCOY1~UJo2vI2rZwnzCKVlV4xfgSEU2SduOLK0TBHKYZGIONdQLCszY1CpWijo3sxqbFgEUCWB6mlgkQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pictures with both the normal and abnormal patterns for the break-apart and fusion strategies.
In all cases the clonal PCs are identified by the intense blue fluorescence of the cytoplasm as detected by the cIg-FISH procedure. (A) A PC with the normal configuration for the VH (red signal) and CH probes (green signal). Arrows point to the closely associated pairs of signals indicative of a normal pattern. (B) Segregation (break-apart) of the VH (green) and CH (red) probes consistent with a 14q32 translocation. (C) A cell with fusion signals (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 4p16.3 (red) consistent with a t(4;14)(p16.3;q32). (D) The fusion signal (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 16q23 (red) consistent with a t(14;16)(q32;q23). (× 100 magnification, Leica DMRXA microscope [Wetzlar, Germany]).
