Expression of the EGFP marker gene in differentiated hematopoietic cells following transplantation of ZAP-70/EGFP–transduced BM cells.

The percentages of transduced splenic T cells, B cells, and myeloid cells, as well as red blood cells (RBCs) and platelets (Plts), were assessed in ZAP-70–deficient mice undergoing transplantation with ZAP-70/EGFP–transduced BM progenitor cells. Splenic T, B, and myeloid cells were gated based on CD3, B220, and Gr-1/Mac-1 expression, respectively (left panel, gated populations are indicated). RBCs and Plts were identified based on specific FSC/SSC profiles as previously described.33 34Expression of EGFP in each lineage was determined in the designated gate, and the percentages of positive cells are indicated (right panel). Representative data from 1 of 3 ZAP-70–transduced mice are shown.