Diverse T-cell repertoire following gene therapy of ZAP-70^−/− mice.

The TCRBV repertoire was assessed by a comparison of TCR CDR3 size distribution (Immunoscope profiles) of splenocytes obtained from WT mice and ZAP-70^−/− mice undergoing transplantation with either ZAP-70–transduced progenitor cells or WT BM. Twenty-four PCR products were generated by reverse transcription with 24 different TCRBV subfamily-specific primers and 1 constant β consensus primer (Cβ), followed by a runoff reaction with a fluorescent Cβ primer. The graphs represent fluorescence intensity in arbitrary units (y-axis) plotted against CDR3 size (x-axis). The size distributions within 19 TCRBV families from 1 of 4 analyzed mice in each of the 3 groups are shown.