Effect of vaccinations with MCP-3 or β-defensin 2 fusions with gp120.

Vaccinations with MCP-3 or β-defensin 2 fusions with gp120 elicit sytemic and mucosal CTL. V-3 loop of HIV-1(isolate 89.6)–specific CTL in spleens (panel A); in Peyer patches (panels B,C) pooled from 3 mice per group immunized with pMCP3gp120, pmDF2βgp120, pMDCgp120, or pgp120. Specific lysis of target cells pulsed with the specific peptide (closed symbols, asterisk) or without peptide (open symbols) is compared. Representative data are shown from 2 independent experiments (3 mice per group).