Need for PGE2 for efficient migration of MoDCs toward CCL19 and CCL21.

MoDCs were matured by treatment with a cocktail of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), sCD40L, or polyI:C in the presence or absence of PGE2 for 48 hours. Unstimulated and stimulated MoDCs were analyzed for their chemotactic response to CCL19 (panel A) or CCL21 (panel B). The data are shown as the mean of duplicate cultures ± SEM and are from 1 representative experiment out of 3 performed. The mean number of spontaneously migrated cells were subtracted from the number of cells that migrated in response to chemokines.