PGE2 modulation of chemokine receptor expression on MoDCs.

MoDCs were matured by treatment with a cocktail of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), sCD40L, or polyI:C in the presence or absence of PGE2. After 48 hours, MoDCs were analyzed by flow cytometry for surface expression of the chemokine receptors CCR5, CXCR4, and CCR7 (black lines). Filled gray histograms represent staining with isotype-matched irrelevant mAbs. The inserted numbers resemble the mean fluorescence intensity by the percentage of positive cells. The data shown are from 1 out of 6 experiments that gave similar results.