PGE2 inhibition of cytokine production by activated MoDCs.

Immature MoDCs were stimulated with a cocktail of proinflammatory cytokines (TNF-α, IL-1β, and IL-6); sCD40L, or polyI:C in the presence (hatched bars) or absence of PGE2 (black bars), and ELISA assays were performed with culture supernatants collected after 48 hours. The detection limit was 10 pg/mL for IL-12p70 (A) and 5 pg/mL for TNF-α (B) and IL-10 (C). The amount of TNF-α was not determined in the culture supernatant of MoDCs matured with a cocktail of proinflammatory cytokines because TNF-α was included in the cocktail. The data are shown as the mean of duplicate measurements ± SEM and are from 1 representative experiment out of 3 performed.