Influence of PGE2 on the expression of CD83, CD86, and CD80 on immature and mature MoDCs.

MoDCs were matured by treatment with a cocktail of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), sCD40L, or polyI:C in the presence or absence of PGE2. After 48 hours, MoDCs were analyzed by flow cytometry for the expression of CD83, CD86, and CD80 (black lines). Filled gray histograms represent staining with isotype-matched irrelevant mAbs. The data shown are from 1 out of 8 experiments that all gave similar results.