Fig. 3.
Expression and activation of STAT5a in transduced cells.
(A) Protein lysates were prepared from CD34+ cells transduced with eGFP, STAT5a, or STAT5aΔ750, and Western blot analysis was performed with an N-terminal antibody against STAT5. (B) Nuclear extracts were made of cells transduced with eGFP, STAT5a, or STAT5aΔ750 after either 15 or 8 days of eosinophil differentiation. Cells were starved of cytokines for 16 hours before stimulation with or without IL-5 for 15 minutes. Nuclear extracts were analyzed with EMSA using a 32P-labeled β-casein probe.
