Neutrophil recruitment to PAG-induced granulomas is dependent on MC-derived TNFα.

(A) Time course of PMN influx after injection of PAG inKit+/+ and Kit^W/Kit^W-vmice. Data were pooled from 10 or more mice per genotype and time point–tested in at least 7 independent experiments. (B) Impaired recruitment of PMNs to CGs inKit^W/Kit^W-v mice is MC dependent. The dermis of Kit^W/Kit^W-v mice was reconstituted with connective tissue–type MCs from normalKit+/+ mice before PAG injection, and PMN numbers were determined 12 hours after injection of PAG. Data were pooled from 8 or more mice per genotype in at least 4 independent experiments. (C) PMN recruitment in cutaneous granuloma formation is strongly dependent on the presence of TNFα. Numbers of infiltrating PMNs were determined in PAG-injected skin of mice deficient in TNFα and in wild-type mice. Data were pooled from 5 or more mice per genotype and time point in 3 independent experiments. (D) PMN recruitment was fully restored in skin of TNF+/+ MC-reconstitutedKit^W/Kit^W-v mice. Before PAG injection, the dermis of Kit^W/Kit^W-vmice was reconstituted with MCs from TNFα+/+ mice or TNFα-deficient mice, and numbers of PMNs were assessed 12 hours thereafter. Data were pooled from 8 or more mice per genotype in 2 independent experiments. All data in Figure 3 are shown as means ± SEMs (× 10⁶ cells/granuloma). For all panels in Figure3, *P < .05, ***P < .005, ***P < .001.