Reversal of cytoadherence in resting and stimulated skin grafts by PpMC-179 in vivo.

IRBCs were allowed to firmly adhere to human microvessels in unstimulated and TNF-α–stimulated skin grafts for 5 to 10 minutes. Then, 2 μM PpMC-179 was administered intravenously. (A) A significant number of adherent IRBCs from P00-24 (n = 13 for before PpMC-179 and 12 for after PpMC-179) and P98-10 (n = 5 for before PpMC-179 and 5 for after PpMC-179) became detached from the human microvessels in unstimulated skin grafts within the first 5 minutes of PpMC-179 administration. **P < .01 compared to baseline. (B) The reversal of IRBC adhesion was associated with an increase in the rolling flux fraction of parasite isolate P98-10. *P < .05 compared to baseline. (C) In TNF-α–stimulated skin grafts, IRBC adhesion of parasite isolates P94-59 (n = 4 for before PpMC-179 and n = 4 for after PpMC-179), P00-24 (n = 14 for before PpMC-179 and 7 for after PpMC-179), and P99-26 (n = 7 for before PpMC-179 and n = 3 for after PpMC-179) was significantly reversed. *P < .05 and ***P < .001 compared to TNF-α. (D) An increase in the mean rolling flux fraction of the 3 isolates was also observed, but it was not statistically significant.