Role of SCF pretreatment on irradiated Ba/F3-Kit cell clonogenicity.

Cells were pretreated (○) or not (●) with SCF (200 ng/mL for 30 minutes) and then seeded in flat-bottom 96-wells plates at a density of 10 cells/well. Cells were irradiated at a dose ranging between 0 and 10 Gy. Colonies were counted after 1 week of culture. Results are the mean ± SD of 3 independent experiments. The insert shows the effect of SCF on apoptosis of irradiated Ba/F3-Kit cells. SCF-stimulated or unstimulated Ba/F3-Kit cells were irradiated at 4 Gy and morphology was examined by fluorescence microscopy after DAPI staining at 24 hours. Results are the mean ± SD of 3 independent experiments (*P < .05).