Steady-state kinetic parameters.

(A) Measurement of steady-state kinetic parameters concerning fibrinopeptide A hydrolysis by WT (●) and Arg67His mutant (▵) thrombin. Continuous lines were drawn according to the best-fit k_cat and K_m values listed in Table 1. (B) Measurement of steady-state kinetic parameters concerning hydrolysis of the PAR-1 38-60 peptide by WT (●) and Arg67His mutant (▵) thrombin. Continuous lines were drawn according to the best-fit k_catand K_m values listed in Table 1. The SE is expressed by the vertical bars.