MBN promoter reporter gene assay in hematopoietic cell lines.

Cells were stimulated with IFN-α (1000 U/mL) or imatinib (1 μM for U937, Raji, Jurkat; 0.2 μM for K562) for 48 hours and harvested for dual luciferase measurement. The results were calculated as the ratio of measured firefly light units (flus) relative to renilla luciferase units (rlus) and presented as fold stimulation compared with the unstimulated control. Each experiment was done at least 3 times. (A) U937 monocytes. (B) Jurkat T cells. (C) Raji B cells. (D) K562 erythroleukemic cells.