MBN transcription in healthy donors and IFN-α–treated patients as assessed by RT-PCR.

The lower blots in each panel display transcription levels of the housekeeping gene β-actin as reference gene for healthy donors and IFN-α patients. MBN⁺ U937 cells served as a positive control cell line. (A) Lack of MBNtranscription in the peripheral blood of healthy donors (1 to 10); positive control (U937), and PCR negative control. (B) MBNtranscript levels of IFN-α–treated CML patients in major or complete cytogenetic remission UPN6-13. Positive and negative controls, U937 and —.