IFN-γ content of BM CD4 and CD8 cells in patients with SAA.

Lymphocytes from 30 previously untreated patients with severe AA and 7 healthy volunteers were surface stained with mAbs to CD4-TC and CD8-PE and intracellularly stained with antibody to IFN-γ–FITC mAb. Patient with SAA received immunosuppressive therapy (either ATG/CSA or cyclophosphamide) and were designated responders or nonresponders. The ratio of the MCF for IFN-γ staining is shown for each group.