IFN-γ intracellular cytokine content in CD8 cells of patients with severe AA before and after successful immunosuppressive treatment.

A subset of 27 patients was tested before and 6 months after treatment with either CTX/CSA or ATG/CSA. In 17 of 27 responding patients, IFN-γ was detectable in CD4 and CD8 cells and declined following treatment. None of the 10 nonresponding patients showed elevated IFN-γ lymphocyte staining prior to therapy. The ratio of the MCF_sample to the MCF_isotype.of staining of CD8 cells before and following treatment is shown.