Comparison of IFN-γ intracellular content in previously untreated responding and unresponding patients with severe AA, and healthy controls.

Lymphocytes from 62 previously untreated patients with severe AA (SAA) and 30 healthy volunteers were surface stained with CD4-TC and CD8-PE mAbs and intracellularly stained with IFN-γ–FITC mAb. Patients with SAA received immunosuppressive therapy (either ATG/CSA or CTX/CSA) and were designated as responders or nonresponders on the basis of improvement of BM function by 6 months following treatment to the point that the patient no longer fulfilled severity criteria. The ratio of the MCF_sample to the MCF_isotype of staining of PB lymphocytes is shown.