Detection of IFN-γ and IL-4 after stimulation of normal lymphocytes.

PB lymphocytes from healthy donors were stimulated with either PMA and calcium ionomycin or with IL-4 and were then surface stained with CD4-TC or CD8-TC mAbs, and finally intracellularly stained with IFN-γ mAb (panel A) or IL-4 mAb (panel B) as described in “Patients, materials, and methods.” Scattergrams demonstrate staining in stimulated lymphocytes, but no staining in unstimulated cells (not treated; NT).