Effect of oxidized EPA on leukocyte rolling and adhesion in mesenteric venules in wild-type and PPARα-deficient mice.

Wild-type or PPARα-deficient mice (PPARα^−/−) were given an intraperitoneal injection of vehicle (Veh) alone, native EPA, or oxidized EPA (oxEPA) 1 hour prior to injection of LPS. Five hours later mice were anesthetized and mesenteric venules were observed. Rolling (A) and adherent leukocytes (B) were determined; n = 5 to 7 for each group of mice. *P < .03 compared with Veh + LPS (wild type) and oxidized EPA + LPS (PPARα^−/−). (C) Representative photographs of leukocytes interacting with the vessel wall (arrows) in LPS-stimulated wild-type and PPARα^−/− mice, after indicated treatments, are shown. Pictures were taken with a × 32 objective.