Fig. 5.
Effect of anti-TF F(ab')2 fragments on Pg 1 and 2 binding and activation on the surfaces of 1-LN cells.
(A) Pg 1 (▴) or Pg 2 (●) (0.2 μM) were added to 1-LN cell monolayers on 96-well culture plates in the absence or presence of increasing concentrations of anti-TF F(ab')2 fragments. (B) Pg 1 (20 nM) and uPA (0.25 nM) were incubated with increasing concentrations of TF isolated from 1-LN cells; 0 nM TF (○), 5 nM TF (●), 10 nM TF (▴). The rate of Pm generation was monitored with the Pm substrate S-2251 for 30 minutes at 37°C using and Anthos Labtec kinetic plate reader.
