Fig. 4.
Activation of Pg 1 by 1-LN cell-surface activators.
Pg 1 (0.2 μΜ) was added to 1-LN cell monolayers on 96-well culture plates, and plasmin generation was monitored with the Pm substrate L-Val-Leu-Lys-pNA (s-2251) for 30 minutes at 37°C using an Anthos Labtec kinetic plate reader. The rate of Pm generation was measured in the absence of any inhibitor of activation (○) and in the presence of 20 μg/mL antitissue-type plasminogen activator IgG (▵), 20 μg/mL anti-uPA (●), or 20 μg/mL anti-TF F(ab')2fragments (▴).
