Inhibition of binding of Pg 1 to immobilized TF.

(A) ¹²⁵I-labeled Pg 1 (0.2 μM) incubated with increasing concentrations of nonlabeled Pg 1 (○) and Pg 2 (●). (B)¹²⁵I-labeled Pg 1 (0.2 μM) incubated with increasing concentrations of factor VIIa (○), 6-AHA (▵), α-MGP (▴), or anti-TF F(ab')2 fragments (●). Bound Pg was quantified as described in “Materials and methods.” Data represent means ± SD from experiments performed in triplicate.