Fig. 10.
Fig. 10. TEL-JAK2(5-19)Tyr314Phe displays impaired Ras activation. / Ba/F3 and the indicated Ba/F3-TEL-JAK2 cell lines were cultured in RPMI containing 1% bovine serum albumin (upper panel) or RMPI supplemented with 10% fetal bovine serum (lower panel). Ba/F3 cells were depleted of cytokine and incubated in the presence or absence of IL-3 prior to lysis. Lysates were mixed with a GST-Raf fusion protein, and an anti-Ras Western blot was performed.

TEL-JAK2(5-19)Tyr314Phe displays impaired Ras activation.

Ba/F3 and the indicated Ba/F3-TEL-JAK2 cell lines were cultured in RPMI containing 1% bovine serum albumin (upper panel) or RMPI supplemented with 10% fetal bovine serum (lower panel). Ba/F3 cells were depleted of cytokine and incubated in the presence or absence of IL-3 prior to lysis. Lysates were mixed with a GST-Raf fusion protein, and an anti-Ras Western blot was performed.

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