The 13α^−/− and 10α^−/−αER lines can differentiate into granulocytic and monocytic cells.

Diff Quick staining of representative cytocentrifuged populations from (A) C/EBPα^−/− fetal livers; (B) 13α^−/− cells; (C) 13α^−/− cells following granulocytic differentiation induced by increased IL-3 and GM-CSF; (D) 13α^−/− cells following macrophage differentiation induced by TPA; (E) line 10α^−/−αER grown in vehicle only; (F) line 10α^−/−αER 6 days after treatment with β-estradiol, which induces nuclear localization of the C/EBPα-ER fusion protein and C/EBPα function7,9; (G) line 10α^−/−αER treated with G-CSF only; and (H) line 10α^−/−αER treated with β-estradiol and G-CSF. Original magnification, × 100.