Flow cytometric analysis of p53 protein expression in case 7 of Table 1.

In an indirect staining protocol, a monoclonal antibody (clone DO-7), which recognizes an epitope comprising amino acids 20-25 of the p53 molecule, was used for detection of p53 expression. An isotypic control was used as the first layer followed by a second layer, FITC-conjugated sheep antimouse IgG F(ab′)₂.