Cell-growth and cell-cycle analysis of leukemic blasts and differentiation of leukemic blasts in liquid suspension culture supplemented with different HGFs.

(A) Cell-growth analysis of leukemic blasts. Leukemic blasts were grown in liquid suspension cultures in the presence of different HGFs. Top panel: (○) control; (●) FL; (■) KL, and (▪) IL-3. Middle panel: (○) Epo, Tpo, M-CSF, and G-CSF; (●) IL-3, GM-CSF, Epo, Tpo, M-CSF, and G-CSF; (■) FL, IL-6, bFGF, IL-3, GM-CSF, Epo, Tpo, M-CSF, and G-CF; (▪) KL, IL-6, bFGF, IL-3, GM-CSF, Epo, Tpo, M-CSF, G-CSF. Bottom panel: (○) FL, IL-3, GM-CSF, Epo, Tpo, M-CSF, and G-CSF; (●) KL, IL-3, GM-CSF, Epo, Tpo, M-CSF, and G-CSF. Mean values of 3 independent experiments are shown. (B) Cell-cycle analysis of leukemic blasts. Leukemic blasts were grown for 5 days in liquid suspension in the presence of different HGFs. (a) Control; (b) KL; (c) FL; (d) Epo, Tpo, M-CSF, and G-CSF; (e) FL, Epo, Tpo, M-CSF, and G-CSF; (f) KL, Epo, Tpo, M-CSF, and G-CSF; (g) IL-3, GM-CSF, Epo, Tpo, M-CSF, and G-CSF; (h) FL, KL, IL-6, and bFGF; (i) KL, IL-6, and bFGF; (l) FL, IL-6, bFGF, IL-3, GM-CSF, Epo, Tpo, M-CSF, and G-CSF; and (m) KL, IL-6, bFGF, IL-3, GM-CSF, Epo, Tpo, M-CSF, and G-CSF. Mean + SD of 3 independent experiments is presented. *P < .05. **P < .01. (C) Differentiation of leukemic blasts in liquid suspension culture supplemented with different HGFs. Cells were identified on cytospins stained with May-Grünwald Giemsa and were classified as blasts, erythroid (E), monocytic (Mo), granulocytic (G), or megakaryocytic (MK) cells. (a) Control; (b) KL; (c) FL, KL, IL-6, and bFGF; (d) FL, IL-6, bFGF, IL-3, Epo, Tpo, M-CSF, and G-CSF; (e) KL, IL-6, bFGF, IL-3, Epo, Tpo, M-CSF, and G-CSF; (f) Epo, Tpo, M-CSF, and G-CSF; (g) KL, Epo, Tpo, M-CSF, and G-CSF; and (h) FL, Epo, Tpo, M-CSF, and G-CSF. A representative experiment is presented.