Binding of platelet-selected Fabs to mod SpA.

Polyclonal Fab preparations derived from the original unselected ITP patient A and patient B Fab/phage display libraries (panel A and B, respectively) and from the libraries after each round of platelet panning were assayed for platelet binding by flow cytometry (circles, right set of axes) and for binding to mod-SpA by ELISA (squares, left set of axes).