TNF-induced permeability across EC monolayers is abrogated by TFPI or anti-TF antibody.

Permeability across functional EC monolayers was determined by measuring absorbance at 620 nm 1 hour following TNF treatment for 90 minutes with or without TFPI (A) or anti-TF antibody (B) as described in “Materials and methods.” There was a significant increase in permeability in the presence of TNF and factor VIII–deficient plasma that was largely abrogated by TF inhibition.